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edta 1x citrate  (Diagnostic BioSystems)

 
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    Diagnostic BioSystems edta 1x citrate
    Edta 1x Citrate, supplied by Diagnostic BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/edta+1x+citrate/edta+1x+citrate/pm40605084-89-7-10
    Average 90 stars, based on 1 article reviews
    edta 1x citrate - by Bioz Stars, 2026-09
    90/100 stars

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    Incubation:

    Article Title: Transmission of amyloid-beta and tau pathologies is associated with cognitive impairments in a primate.
    Article Snippet: .. For the HLA-DR and AT100 stainings, the pretreatment consisted in a 30 min-long incubation in EDTA 1X citrate (Diagnostic BioSystems®) at 95 °C followed by rinsings in 0.5% Triton X-100/0.05 M Tris–HCl Buffered Saline solution (TBS) or a PBS solution respectively, for 2 × 10 min. For the 4G8, Aβ42, AT8, AT100, pS422 and HLA-DR stainings, tissues were then incubated in hydrogen peroxide H2O2 30% (Sigma-Aldrich®) diluted 1/100 for 20 min to inhibit endogenous peroxidases. .. Blocking of non-specific antigenic sites was achieved over 1 h using a 0.2% Triton X-100/0.1 M PBS (Sigma-Aldrich®) (PBST) or 0.5% Triton X-100/0.05 M TBS (TBST) solution containing 3% bovine serum albumin or 4.5% normal goat serum, depending on the staining.

    Saline:

    Article Title: Transmission of amyloid-beta and tau pathologies is associated with cognitive impairments in a primate.
    Article Snippet: .. For the HLA-DR and AT100 stainings, the pretreatment consisted in a 30 min-long incubation in EDTA 1X citrate (Diagnostic BioSystems®) at 95 °C followed by rinsings in 0.5% Triton X-100/0.05 M Tris–HCl Buffered Saline solution (TBS) or a PBS solution respectively, for 2 × 10 min. For the 4G8, Aβ42, AT8, AT100, pS422 and HLA-DR stainings, tissues were then incubated in hydrogen peroxide H2O2 30% (Sigma-Aldrich®) diluted 1/100 for 20 min to inhibit endogenous peroxidases. .. Blocking of non-specific antigenic sites was achieved over 1 h using a 0.2% Triton X-100/0.1 M PBS (Sigma-Aldrich®) (PBST) or 0.5% Triton X-100/0.05 M TBS (TBST) solution containing 3% bovine serum albumin or 4.5% normal goat serum, depending on the staining.



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